Diana Yamile Gallego Villa, Anna Lisa Piccinelli, Luigi Russo, Luca Rastrelli
*1 Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Cra 30 Nº 45-03, Bogotá, Colombia
2 Dipartimento di Farmacia, University of Salerno, Via Ponte don Melillo, 84084, Fisciano (SA), Italy
Abstract
Cottonseeds are fed to high-producing dairy cows as a source of fat and highly-digestible fiber. Previously we reported the isolation and structure characterization of different phenolic compounds, quercetin, kaemferol and benzoic acid derivatives (1-9) from whole cottonseed by-product. In this paper the antioxidant activity of all isolated compounds was determined by measuring free radical scavenging effects using the Trolox equivalent antioxidant capacity assay and the coupled oxidation of β-carotene and linoleic acid (autoxidation assay). The range of phenolic compounds present in WCS (1.90 g/kg), assayed by the Folin-Ciocalteu method, and their antioxidant properties provides a valuable database for possible nutritional studies on the impact of diet on animal health and productivity.
Keywords: Whole cottonseed, By-products, Flavonol glycosides, Benzoic acid derivatives, Antioxidant activity, Feedstuff